TNNI3 (Phospho-Thr142) Antibody

Catalog Number:

12028

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P19429;
NCBI Gene#: 7137;
NCBI Protein#: NP_000354.4

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Threonine 142 (R-P-T(p)-L-R) derived from Human TNNI3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of TNNI3 only when phosphorylated at Threonine 142.

Applications:

WB

Background:

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

References:

appl_detail:

Predicted MW: 28kd
Western blotting: 1:500~1:1000

Datasheet PDF