TK (Phospho-Ser13) Antibody

Catalog Number:

12027

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P04183;
NCBI Gene#: 7083;
NCBI Protein#: XP_005257688.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 13(P-G-S(p)-P-S) derived from Human TK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of TK only when phosphorylated at Serine 13.

Applications:

WB

Background:

TK is a cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

References:

appl_detail:

Predicted MW: 25kd
Western blotting: 1:500~1:1000

Datasheet PDF