MITF (Phospho-Ser180/73) Antibody

Catalog Number:

12018

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O75030;
NCBI Gene#: 4286;
NCBI Protein#: XP_005264811.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 180(P-N-S(p)-P-M)/Serine 73(Q-E-R(p)-R-E) derived from Human MITF.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of MITF only when phosphorylated at Serine 180/73.

Applications:

WB

Background:

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

References:

appl_detail:

Predicted MW: 52kd
Western blotting: 1:500~1:1000

Datasheet PDF