IGFBP-3 (Phospho-Ser183) Antibody

Catalog Number:

11999

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P17936;
NCBI Gene#: 3486;
NCBI Protein#: NP_000589.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 183(K-D-S(p)-Q-R) derived from Human IGFBP-3 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IGFBP-3 only when phosphorylated at serine 183.

Applications:

WB

Background:

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Zhang Q, Steinle JJ (2013)Invest Ophthalmol Vis Sci 54, 3052-7 . Cobb LJ, Liu B, Lee KW, Cohen P (2006) Cancer Res 66, 10878-84 .

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF