Cyclin D1 (Phospho-Ser90) Antibody

Catalog Number:

11986

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P24385;
NCBI Gene#: 595;
NCBI Protein#: NP_444284.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 90(F-L-S(p)-L-E) derived from Human Cyclin D1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Cyclin D1 only when phosphorylated at serine 90.

Applications:

WB

Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.

Sewing A, M¨¹ller R (1994) Oncogene 9, 2733-6

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF