HDAC6 (Phospho-Ser22) Antibody

Catalog Number:

11984

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9UBN7;
NCBI Gene#: 10013;
NCBI Protein#: NP_006035.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 22 (P-Q-S(p)-P-P) derived from Human HDAC6.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of HDAC6 only when phosphorylated at serine 22.

Applications:

WB

Background:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes By similarity. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

Chen S, Owens GC, Makarenkova H, Edelman DB (2010)PLoS One 5, e10848.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF