GTPase Activating Protein (Phospho-Ser387) Antibody

Catalog Number:

11983

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9H0H5;
NCBI Gene#: 29127;
NCBI Protein#: NP_001119575.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 387 (R-I-S(p)-G-C) derived from Human GTPase Activating Protein.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of GTPase Activating Protein only when phosphorylated at serine 387.

Applications:

WB

Background:

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation.

Doki N, et al. (2009)Cancer Sci 100, 1675-9. Minoshima Y, et al. (2003)Dev Cell 4, 549-60.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF