AurB (Phospho-Thr232) Antibody

Catalog Number:

11982

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q96GD4;
NCBI Gene#: 9212;
NCBI Protein#: NP_001243763.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 232 (R-K-T(p)-M-C) derived from Human AurB.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of AurB only when phosphorylated at threonine 232.

Applications:

WB

Background:

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.

Caldas GV, DeLuca KF, DeLuca JG (2013)J Cell Biol 203, 957-69. Zhang L, et al. (2012)J Biol Chem 287, 34069-77. Ratushny V, et al. (2012)Oncogene 31, 1217-27 .

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF