CaMK4 (Phospho-Thr200) Antibody

Catalog Number:

11981

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q16566;
NCBI Gene#: 814;
NCBI Protein#: NP_001735.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 200 (M-K-T(p)-V-C) derived from Human CaMK4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CaMK4 only when phosphorylated at threonine 200.

Applications:

WB

Background:

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2).

Oury F, et al. (2010)Genes Dev 24, 2330-42. Dias WB, Cheung WD, Wang Z, Hart GW (2009) J Biol Chem 284, 21327-37. Chow FA, Anderson KA, Noeldner PK, Means AR (2005)J Biol Chem 280, 20530-8.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF