p70 S6 Kinase (Phospho-Thr389/412) Antibody

Catalog Number:

11974

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P23443;
NCBI Gene#: 6198;
NCBI Protein#: NP_001258971.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine389/412(G-F-T(p)-Y-V) derived from Human p70 S6 Kinase .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412.

Applications:

WB

Background:

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

Hong S, et al. (2014) J Biol Chem
Puustinen P, et al. (2014) CJ Cell Biol 204, 713-27
Martineau Y, et al. (2014) Mol Cell Biol 34, 1046-53

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF