Bcr (Phospho-Tyr360) Antibody

Catalog Number:

11972

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11274;
NCBI Gene#: 613;
NCBI Protein#: NP_004318.3

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 360 (T-T-Y(p)-R-M) derived from Human Bcr.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 360.

Applications:

WB

Background:

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Perazzona B, et al. (2008) Oncogene 27, 2208-14. Sun T, Campbell M, Gordon W, Arlinghaus RB (2001) Biopolymers 60, 61-75 . Wu Y, Liu J, Arlinghaus RB (1998)Oncogene 16, 141-6.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF