c-Met (Phospho-Tyr1003) Antibody

Catalog Number:

11971

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P08581;
NCBI Gene#: 4233;
NCBI Protein#: NP_000236.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1003 (V-D-Y(p)-R-A) derived from Human c-Met.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of c-Met only when phosphorylated at tyrosine 1003.

Applications:

WB

Background:

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.

Usatyuk PV, et al. (2014)J Biol Chem. Asiedu MK, et al. (2014) Oncogene 33, 1316-24. Dulak AM, et al. (2011) Oncogene 30, 3625-35.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF