p53 (Phospho-Ser392) Antibody

Catalog Number:

11969

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P04637;
NCBI Gene#: 7157;
NCBI Protein#: NP_000537.3

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 392 (P-D-S(p)-D) derived from Human p53.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of p53 only when phosphorylated at serine 392.

Applications:

WB

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Choi DW, et al. (2013) Mol Cell 51, 374-85
Bagashev A, et al. (2013) Cell Cycle 12, 1569-77
Shahar OD, et al. (2013) PLoS One 8, e78472

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF