PDCD4 (Phospho-Ser457) Antibody

Catalog Number:

11962

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q53EL6;
NCBI Gene#: 27250;
NCBI Protein#: NP_001186421.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 457 (F-V-S(p)-E-G) derived from Human PDCD4 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PDCD4 only when phosphorylated at serine 457.

Applications:

WB

Background:

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA

Kakade D, Islam N, Maeda N, Adegoke OA (2014) BMC Cell Biol 15, 2. Wippich F, et al. (2013)Cell 152, 791-805 . LaRonde-LeBlanc N, et al. (2007)Mol Cell Biol 27, 147-56.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF