Pin1 (Phospho-Ser16) Antibody

Catalog Number:

11960

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13526;
NCBI Gene#: 5300;
NCBI Protein#: NP_006212.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine16(R-M-S(p)-R-S) derived from Human Pin1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Pin1 only when phosphorylated at serine 16.

Applications:

WB

Background:

Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.

Lee YC, et al. (2013)J Cell Sci 126, 4862-72. Ando K, et al. (2013)Neurobiol Aging 34, 757-69. Nechama M, Lin CL, Richter JD (2013)Mol Cell Biol 33, 48-58.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF