XIAP (Phospho-Ser87) Antibody

Catalog Number:

11956

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P98170;
NCBI Gene#: 331;
NCBI Protein#: NP_001158.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine87(K-V-S(p)-P-N) derived from Human XIAP .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of XIAP only when phosphorylated at serine 87.

Applications:

WB

Background:

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state.

Huang X, Wu Z, Mei Y, Wu M (2013)EMBO J 32, 2204-16. Bornhauser BC, et al. (2007) Blood 110, 2084-91.Dan HC, et al. (2004)J Biol Chem 279, 5405-12.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF