YAP (Phospho-Ser127) Antibody

Catalog Number:

11952

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P46937;
NCBI Gene#: 10413;
NCBI Protein#: NP_001123617.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of YAP only when phosphorylated at serine 127.

Applications:

WB

Background:

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.

Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66
Yang S, et al. (2014) Cell Signal 26, 343-51
Sun W, et al. (2014) Mol Biol Rep 41, 947-56

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF