SREBP-1 (Phospho-Ser439) Antibody

Catalog Number:

11951

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P36956;
NCBI Gene#: 6720;
NCBI Protein#: NP_001005291.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine439(Q-S-S(p)-P-L) derived from Human SREBP-1 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of SREBP-1 only when phosphorylated at serine 439.

Applications:

WB

Background:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway By similarity. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

Bengoechea-Alonso MT, Ericsson J (2006) Cell Cycle 5, 1708-18

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF