VE-Cadherin (Phospho-Tyr731) Antibody

Catalog Number:

11950

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P33151;
NCBI Gene#: 1003;
NCBI Protein#: NP_001786.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine731 (H-I-Y(p)-G-Y) derived from Human VE-Cadherin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of VE-Cadherin only when phosphorylated at tyrosine 731.

Applications:

WB

Background:

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen.

Wessel F, et al. (2014) Nat Immunol 15, 223-30
Sawada J, et al. (2012) Cancer Cell 22, 235-49
Turowski P, et al. (2008) J Cell Sci 121, 29-37

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF