CDC25B (Phospho-Ser353) Antibody

Catalog Number:

11949

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30305;
NCBI Gene#: 994;
NCBI Protein#: NP_001274445.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CDC25B only when phosphorylated at serine 353.

Applications:

WB

Background:

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Reboutier D, et al. (2012) J Cell Biol 197, 19-26
Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90
Feng C, et al. (2007) Biol Reprod 77, 560-8

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF