CBL (Phospho-Tyr674) Antibody

Catalog Number:

11946

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P22681;
NCBI Gene#: 867;
NCBI Protein#: NP_005179.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine674 (A-I-Y(p)-S-L) derived from Human CBL.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of CBL only when phosphorylated at tyrosine 674.

Applications:

WB

Background:

Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

Fernandes MS, et al. (2010) J Biol Chem 285, 32596-605
Teckchandani AM, Panetti TS, Tsygankov AY (2005) Exp Cell Res 307, 247-58

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF