Src (Phospho-Ser75) Antibody

Catalog Number:

11944

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P12931;
NCBI Gene#: 6714;
NCBI Protein#: NP_005408.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 75 (V-T-S(p)-P-Q) derived from Human Src.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Src only when phosphorylated at serine 75.

Applications:

WB

Background:

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pan Q, et al. (2011)Cell Mol Life Sci 68, 3425-36 . Kato G, Maeda S (2003) J Biochem (Tokyo) 133, 563-9. Kato G, Maeda S (1999)J Biochem (Tokyo) 126, 957-61.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF