PDGFR beta (Phospho-Tyr740) Antibody

Catalog Number:

11941

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P09619;
NCBI Gene#: 5159;
NCBI Protein#: NP_002600.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 740 (G-G-Y(p)-M-D) derived from Human PDGFR beta.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PDGFR beta only when phosphorylated at tyrosine 740.

Applications:

WB

Background:

PDGF Receptor β encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Mitchell D, et al. (2007) J Biol Chem 282, 15606-18Wu JH, et al. (2006) J Biol Chem 281, 37758-72 . Bae YS, et al. (2000)J Biol Chem 275, 10527-31.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF