IR (Phospho-Tyr1355) Antibody

Catalog Number:

11939

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P06213;
NCBI Gene#: 3643;
NCBI Protein#: NP_000199.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1355 (R-S-Y(p)-E-E) derived from Human IR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IR only when phosphorylated at tyrosine 1355.

Applications:

WB

Background:

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Tennagels N, et al. (2001) Biochem Biophys Res Commun 282, 387-93
Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) FEBS Lett 479, 67-71
Noelle V, Tennagels N, Klein HW (2000) Biochemistry 39, 7170-7

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF