ATPase (Phospho-Ser16) Antibody

Catalog Number:

11938

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P05023;
NCBI Gene#: 476;
NCBI Protein#: NP_000692.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 16 (A-V-S(p)-E-Q) derived from Human ATPase.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of ATPase only when phosphorylated at serine 16.

Applications:

WB

Background:

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Kava L, Rossi NF, Mattingly R, Yingst DR (2012) Am J Hypertens 25, 487-91
Soltoff SP, Asara JM, Hedden L (2010)J Biol Chem 285, 36330-8
Efendiev R, et al. (2006) FEBS Lett 580, 5067-70

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF