GR (Phospho-Ser226) Antibody

Catalog Number:

11937

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P04150;
NCBI Gene#: 2908;
NCBI Protein#: NP_000167.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 226 (L-L-S(p)-P-L) derived from Human GR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of GR only when phosphorylated at serine 226.

Applications:

WB

Background:

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.

Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) J Biol Chem 289, 10235-51
Bouazza B, et al. (2014) Am J Respir Cell Mol Biol 50, 301-15
Lee MS, et al. (2013) Behav Brain Res 236, 56-61

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF