IKK-γ (Phospho-Ser85) Antibody

Catalog Number:

11927

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9Y6K9;
NCBI Gene#: 8517;
NCBI Protein#: NP_001093326.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 85 (Q-A-S(p)-Q-R) derived from Human IKK-gamma.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IKK-γ only when phosphorylated at serine 85.

Applications:

WB

Background:

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.

Niu J, Shi Y, Iwai K, Wu ZH (2011) EMBO J 30, 3741-53. Wu ZH, et al. (2010)Mol Cell 40, 75-86. Palkowitsch L, Leidner J, Ghosh S, Marienfeld RB (2008)J Biol Chem 283, 76-86.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF