SirT1 (Phospho-Ser47) Antibody

Catalog Number:

11926

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q96EB6;
NCBI Gene#: 23411;
NCBI Protein#: NP_001135970.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 47(E-R-S(p)-P-G) derived from Human SirT1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of SirT1 only when phosphorylated at serine 47.

Applications:

WB

Background:

NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively.

Gao Z, et al. (2011) J Biol Chem 286, 22227-34. Zschoernig B, Mahlknecht U (2009)Biochem Biophys Res Commun 381, 372-7. Nasrin N, et al. (2009)PLoS One 4, e8414.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF