Smad2 (Phospho-Ser250) Antibody

Catalog Number:

11924

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q15796;
NCBI Gene#: 4087;
NCBI Protein#: NP_001003652.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine250(E-L-S(p)-P-T) derived from Human Smad2 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Smad2 only when phosphorylated at serine 250.

Applications:

WB

Background:

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Fransvea E, Mazzocca A, Antonaci S, Giannelli G (2009)Hepatology 49, 839-50 . Funaba M, Zimmerman CM, Mathews LS (2002)J Biol Chem 277, 41361-8. Kretzschmar M, Doody J, Timokhina I, Massagu¨¦ J (1999)Genes Dev 13, 804-16 .

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF