Smad3 (Phospho-Ser204) Antibody

Catalog Number:

11916

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P84022;
NCBI Gene#: 4088;
NCBI Protein#: NP_001138574.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine204(A-G-S(p)-P-N) derived from Human Smad3 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Smad3 only when phosphorylated at serine 204.

Applications:

WB

Background:

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Cohen-Solal KA, et al. (2011) Pigment Cell Melanoma Res 24, 512-24
Seong HA, Jung H, Ha H (2010) J Biol Chem 285, 30959-70
Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol Cancer Res 8, 1375-87

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF