PDGFRa (Phospho-Tyr849) Antibody

Catalog Number:

11912

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P16234;
NCBI Gene#: 5156;
NCBI Protein#: NP_006197.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine849(S-N-Y(p)-V-S) derived from Human PDGFRa.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PDGFRa only when phosphorylated at tyrosine 849.

Applications:

WB

Background:

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi.

Rikova K, et al. (2007) Cell 131, 1190-203
Moritz A, et al. (2010) Sci Signal 3, ra64
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF