Ezrin (Phospho-Tyr146) Antibody

Catalog Number:

11909

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P15311;
NCBI Gene#: 7430;
NCBI Protein#: NP_001104547.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 146 (S-G-Y(p)-L-S) derived from Human Ezrin.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 146.

Applications:

WB

Background:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Murchie R, et al. (2014)Proc Natl Acad Sci U S A 111, 693-8. Kawahara T, et al. (2013)PLoS One 8, e79654 . Wang Y, Lazo JS (2012)Oncogene 31, 818-27.

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF