PKCB (Phospho-Ser661) Antibody

Catalog Number:

11905

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P05771;
NCBI Gene#: 5579;
NCBI Protein#: NP_002729.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 661 (G-F-S(p)-Y-T) derived from Human PKCB.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of PKCB only when phosphorylated at serine 661.

Applications:

WB

Background:

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.

Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13
Leonard TA, et al. (2011) Cell 144, 55-66
Qiao M, et al. (2010) Mol Cell 38, 512-23

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF