Trk A (Phospho-Tyr680/681) Antibody

Catalog Number:

11904

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P04629;
NCBI Gene#: 4914;
NCBI Protein#: NP_001007793.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 680/tyrosine 681 (T-D-Y(p)-Y(p)-R-V) derived from Human Trk A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Trk A only when phosphorylated at tyrosine 680/tyrosine 681.

Applications:

WB

Background:

Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.

Liu HY, et al. (2005) J Biol Chem 280, 19461-71
Wahab NA, Weston BS, Mason RM (2005) J Am Soc Nephrol 16, 340-51
Marsh HN, et al. (2003) J Cell Biol 163, 999-1010

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF