Abl (Phospho-Tyr204) Antibody

Catalog Number:

11901

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P00519;
NCBI Gene#: 25;
NCBI Protein#: NP_005148.2

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 204 (K-L-Y(p)-V-S) derived from Human c-Abl.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Abl only when phosphorylated at tyrosine 204.

Applications:

WB

Background:

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins).

Meyn MA, et al. (2006) J Biol Chem 281, 30907-16
Steen H, et al. (2003) Mol Cell Proteomics 2, 138-45
Salomon AR, et al. (2003) Proc Natl Acad Sci U S A 100, 443-8

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF