MAP3K7 (Phospho-Thr187) Antibody

Catalog Number:

11899

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O43318;
NCBI Gene#: 6885;
NCBI Protein#: NP_003179.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 187 (H-M-T(p)-N-N) derived from Human MAP3K7.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of IMAP3K7 only when phosphorylated at threonine 187.

Applications:

WB

Background:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.

Shin MS, et al. (2009)Biochim Biophys Acta 1793, 1156-64
Liu Q, Busby JC, Molkentin JD (2009)Nat Cell Biol 11, 154-61
Kim SI, Kwak JH, Wang L, Choi ME (2008)J Biol Chem 283, 10753-63

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF