Epo-R (Phospho-Tyr368) Antibody

Catalog Number:

11996

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P19235;
NCBI Gene#: 2057;
NCBI Protein#: NP_000112.1

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Tyrosine 368 (D-T-Y(p)-L-V) derived from Human Epo-R.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous level of Epo-R only when phosphorylated at tyrosine 368.

Applications:

WB

Background:

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

Funakoshi-Tago M, et al. (2010) J Biol Chem 285, 5296-307
Sulahian R, Cleaver O, Huang LJ (2009) Blood 113, 5287-97
Zhang YL, et al. (2009) Mol Cell 33, 266-74

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF