BRCA2 (Phospho-Ser3291) Antibody

Catalog Number:

11840

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P51587;
NCBI Gene#: 675;
NCBI Protein#: NP_000050.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 3291 (F-V-S(p)-P-A) derived from Human BRCA2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291.

Applications:

WB

Background:

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.

Wooster R., Nature 378:789-792(1995).
Tavtigian S.V., Nat. Genet. 12:333-337(1996).
Dunham A., Nature 428:522-528(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF