MuSK (Phospho-Tyr755) Antibody

Catalog Number:

11837

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O15146;
NCBI Gene#: 4593;
NCBI Protein#: NP_005583.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 755(A-D-Y(p)-Y-K) derived from Human MuSK .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MuSK only when phosphorylated at tyrosine 755.

Applications:

WB

Background:

Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane.

Valenzuela D.M., Neuron 15:573-584(1995).
Humphray S.J., Nature 429:369-374(2004).
Bergamin E., Mol. Cell 39:100-109(2010).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF