EPHA2/5 (Phospho-Tyr594) Antibody

Catalog Number:

11833

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P29317/P54756;
NCBI Gene#: 1969/2044;
NCBI Protein#: NP_004422.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 594 (H-T-Y(p)-E-D) derived from Human EPHA2/5.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The Antibody detects endogenous levels of EPHA2 only when phosphorylated at tyrosine 594.

Applications:

WB

Background:

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway.

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990).
Gregory S.G., Nature 441:315-321(2006).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF