VAV3 (Phospho-Tyr173) Antibody

Catalog Number:

11830

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9UKW4;
NCBI Gene#: 10451;
NCBI Protein#: NP_006104.4.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 173 (E-V-Y(p)-E-D) derived from Human VAV3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of VAV3 only when phosphorylated at tyrosine 173.

Applications:

WB

Background:

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Trenkle T., Nucleic Acids Res. 26:3883-3891(1998).
Movilla N., Mol. Cell. Biol. 19:7870-7885(1999).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF