TUBA1/3/4 (Phospho-Tyr272) Antibody

Catalog Number:

11829

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366;
NCBI Gene#: 7846/10376/84790/113457/7278/112714/7277;
NCBI Protein#: NP_001257328.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at tyrosine 272.

Applications:

WB

Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.

Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001).
Sugano S., Nat. Genet. 36:40-45(2004).
Venter J.C., Submitted (JUL-2005).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF