SYK (Phospho-Tyr352) Antibody

Catalog Number:

11827

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P43405;
NCBI Gene#: 6850;
NCBI Protein#: NP_001167638.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 352(S-P-Y(p)-A-D) derived from Human SYK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of SYK only when phosphorylated at tyrosine 352.

Applications:

WB

Background:

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

Yagi S., Biochem. Biophys. Res. Commun. 200:28-34(1994).
Law C.-L., J. Biol. Chem. 269:12310-12319(1994).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF