MAPK1/3 (Phospho-Tyr205/222) Antibody

Catalog Number:

11825

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P28482/P27361;
NCBI Gene#: 5594/5595;
NCBI Protein#: NP_002736.3.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 205/222(K-G-Y(p)-T-K) derived from Human MAPK1/3 .

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MAPK1/3 only when phosphorylated at tyrosine 205/222.

Applications:

WB

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992).
Dunham I., Nature 402:489-495(1999).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF