YB1 (Phospho-Ser102) Antibody

Catalog Number:

11819

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P67809;
NCBI Gene#: 4904;
NCBI Protein#: NP_004550.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 102(L-R-S(p)-V-G) derived from Human YB1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of YB1 only when phosphorylated at serine 102.

Applications:

WB

Background:

YB-1 is a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP.

Sakura H., Gene 73:499-507(1988).
Didier D.K. Proc. Natl. Acad. Sci. U.S.A. 85:7322-7326(1988).
Kolluri R., Nucleic Acids Res. 19:4771-4771(1991).

References:

appl_detail:

Western blotting: 1:500~1:1000

Datasheet PDF