TOB1 (Phospho-Ser164) Antibody

Catalog Number:

11816

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P50616;
NCBI Gene#: 10140;
NCBI Protein#: NP_001230806.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

Applications:

WB IHC

Background:

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

Matsuda S., Oncogene 12:705-713(1996).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF