MAPKAPK2 (Phospho-Ser272) Antibody

Catalog Number:

11806

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P49137;
NCBI Gene#: 9261;
NCBI Protein#: NP_116584.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 272(A-I-S(p)-P-G) derived from Human MAPKAPK2.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of MAPKAPK2 only when phosphorylated at serine 272.

Applications:

WB IHC

Background:

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Zu Y.-L., Biochem. Biophys. Res. Commun. 200-1118-1124(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Stokoe D., Biochem. J. 296:843-849(1993).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF