Cyclin C (Phospho-Ser275) Antibody

Catalog Number:

11797

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P24863;
NCBI Gene#: 892;
NCBI Protein#: NP_005181.2.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Cyclin C only when phosphorylated at serine 275.

Applications:

WB IHC

Background:

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.

Lew D.J., Cell 66:1197-1206(1991).
Li H., Genomics 32:253-259(1996).
Mungall A.J., Nature 425:805-811(2003).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF