CtBP1 (Phospho-Ser422) Antibody

Catalog Number:

11796

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q13363;
NCBI Gene#: 1487;
NCBI Protein#: NP_001319.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of Serine 422(A-P-S(p)-P-G) derived from Human CtBP1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422.

Applications:

WB IHC

Background:

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Schaeper U., Proc. Natl. Acad. Sci. U.S.A. 92:10467-10471(1995).
Sewalt R.G.A.B., Mol. Cell. Biol. 19:777-787(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF