Claudin 5 (Phospho-Tyr217) Antibody

Catalog Number:

11792

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O00501;
NCBI Gene#: 7122;
NCBI Protein#: NP_001124333.1.

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 217 (K-K-N-Y(p)-V) derived from Human Claudin 5.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Specificity/Sensitivity:

The antibody detects endogenous levels of Claudin 5 only when phosphorylated at tyrosine 217.

Applications:

IHC

Background:

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome.

Sirotkin H., Genomics 42:245-251(1997).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

Datasheet PDF